Gibberellin 3-oxidase Gene Expression Patterns Influence Gibberellin Biosynthesis, Growth, and Development in Pea
نویسندگان
چکیده
منابع مشابه
Sites of gibberellin biosynthesis in pea seedlings.
Potential sites of gibberellin biosynthesis in 10-day-old ;Alaska' pea (Pisum sativum L.) seedlings were investigated using a cell-free ezyme system capable of incorporating [(14)C]-mevalonic acid into ent-kaurene. In peas, ent-kaurene is assumed to be a committed intermediate in the gibberellin biosynthetic pathway. Comparative results from enzyme assays using extracts from shoot tips, leaf bl...
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To elucidate the involvement of gibberellin (GA) in the growth regulation of Arabidopsis roots, effects of shoot-applied GA and GA-biosynthesis inhibitors on the root were examined. Applying GA to the shoot of Arabidopsis slightly enhanced the primary root elongation. Treating shoots with uniconazole, a GA biosynthesis inhibitor, also resulted in enhancement of primary root elongation, while sh...
متن کاملCloning and overproduction of gibberellin 3-oxidase in hybrid aspen trees. Effects on gibberellin homeostasis and development.
To broaden our understanding of gibberellin (GA) biosynthesis and the mechanism whereby GA homeostasis is maintained in plants, we have investigated the degree to which the enzyme GA 3-oxidase (GA3ox) limits the formation of bioactive GAs in elongating shoots of hybrid aspen (Populus tremula x Populus tremuloides). We describe the cloning of a hybrid aspen GA3ox and its functional characterizat...
متن کاملDevelopmental and embryo axis regulation of gibberellin biosynthesis during germination and young seedling growth of pea.
The expression patterns of five genes (PsGA20ox1, PsGA20ox2, PsGA3ox1, PsGA2ox1, and PsGA2ox2) encoding five regulatory gibberellin (GA) biosynthesis enzymes (two GA 20-oxidases, a GA 3beta-hydroxylase, and two GA 2beta-hydroxylases) were examined to gain insight into how these genes coordinate GA biosynthesis during germination and early postgermination stages of the large-seeded dicotyledonou...
متن کاملArabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ...
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ژورنال
عنوان ژورنال: PLANT PHYSIOLOGY
سال: 2013
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.113.225987